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KMID : 0380219930260040359
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Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 4 p.359 ~ p.362
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Phosphorylated Intermediate of Hepatic Glucose-6-Phosphatase
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Jung-A Oh and Jong-Sang Park
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Abstract
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Glucose-6-phosphatase is an important enzyme involved in the regulation of concentration of glucose in blood. Steady-state labeling, a method to incorporate ^32 to the active site of Glucose-6-Phosphatase, was employed to identify the target enzyme. By the steady state labeling about 62-kDa protein was labeled in the microsome from rat liver. The 62-kDa band disappeared in the presence of known inhibitors and competitors of glucose-6-phosphatase. 35-kDa protein was digested protein fragment from the 62-kDa protein. These results showed that the 62-kDa protein was the phosphohydrolase component of glucose-6-phosphatase system.
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